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KMID : 1134820080370081009
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Journal of the Korean Society of Food Science and Nutrition
2008 Volume.37 No. 8 p.1009 ~ p.1017
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Fractionation of Exopeptidase from Viscera of Argentina Shortfin Squid, Illex argentinus
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Kim Hye-Suk
Kim Jin-Soo
Heu Min-Soo
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Abstract
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For the effective use of exopeptidase from squid viscera as food processing aids, the viscera of Argentina
shortfin squid (Illex argentinus) were fractionated by various methods such as acetone treatment, ammonium
sulfate treatment, anion exchange chromatography, and gel filtration. The positive exopeptidase fractions were
obtained from the fraction Ⅱ treated by cold acetone (30～40%, w/w), the fraction Ⅴ by ammonium sulfate
(60～70% saturation), the fraction Ⅱ (0.2 M NaCl) by anion exchange chromatography, and the fraction Ⅰ
(30～50 kDa) by gel filtration. The specific activities of positive fractions from viscera of Illex argentinus
against substrates were higher to LeuPNA than to ArgPNA. Total activity and recovery against LeuPNA of
positive fraction by gel filtration were 1,867 U and 30.69%, respectively, which were the highest among those
of positive fraction. The results suggested that the gel filtration chromatography method was the most efficient
method for the fractionation of exopeptidase from viscera of Illex argentinus.
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KEYWORD
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squid, squid viscera, exopeptidase, Illex argentinus, squid by-products
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